In vitro effects of cadmium on two different animal cell models
Identifieur interne : 000243 ( Main/Exploration ); précédent : 000242; suivant : 000244In vitro effects of cadmium on two different animal cell models
Auteurs : I. Olabarrieta [Espagne] ; B. L'Azou [France] ; S. Yuric [France] ; J. Cambar [France] ; M.P Cajaraville [Espagne]Source :
- Toxicology in Vitro [ 0887-2333 ] ; 2001.
Abstract
The aim of this study was to assess comparatively the effects of cadmium on two different in vitro cell models, a cell line derived from proximal tubule renal cells (LLC-PK1) and haemocytes or blood cells of mussels (Mytilus galloprovincialis). Cells were seeded in 96-well microplates and exposed in vitro to different concentrations of cadmium (CdCl2) ranging from 10 to 2000 μm for haemocytes and from 1 to 100 μm for LLC-PK1 cells, added to the culture medium. After 24 h of exposure, different assays were performed on haemocytes: neutral red uptake, phagocytosis of neutral red-stained zymosan, XTT assay, activity of lysosomal acid phosphatase and demonstration of the actin cytoskeleton using TRITC-labeled phalloidin. Cell viability expressed as LC50 was 750 μm when using the neutral red assay and 400 μm with the XTT assay. The phagocytic ability and the activity of acid phosphatase increased significantly in cells treated with Cd in a non dose-dependent manner. Doses of Cd above 100 μm caused disruption of the actin cytoskeleton. In LLC-PK1 cells, cell viability expressed as LC50 was found to be around 40 μm when using the neutral red assay and 50–60 μm with MTT and LDH assays, respectively. These results show that mussel haemocytes are in general more resistant to Cd exposure than LLC-PK1 cells. Furthermore, Cd appears to stimulate phagocytic and lysosomal activities in haemocytes in vitro.
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DOI: 10.1016/S0887-2333(01)00056-X
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Cells were seeded in 96-well microplates and exposed in vitro to different concentrations of cadmium (CdCl2) ranging from 10 to 2000 μm for haemocytes and from 1 to 100 μm for LLC-PK1 cells, added to the culture medium. After 24 h of exposure, different assays were performed on haemocytes: neutral red uptake, phagocytosis of neutral red-stained zymosan, XTT assay, activity of lysosomal acid phosphatase and demonstration of the actin cytoskeleton using TRITC-labeled phalloidin. Cell viability expressed as LC50 was 750 μm when using the neutral red assay and 400 μm with the XTT assay. The phagocytic ability and the activity of acid phosphatase increased significantly in cells treated with Cd in a non dose-dependent manner. Doses of Cd above 100 μm caused disruption of the actin cytoskeleton. In LLC-PK1 cells, cell viability expressed as LC50 was found to be around 40 μm when using the neutral red assay and 50–60 μm with MTT and LDH assays, respectively. These results show that mussel haemocytes are in general more resistant to Cd exposure than LLC-PK1 cells. Furthermore, Cd appears to stimulate phagocytic and lysosomal activities in haemocytes in vitro.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Bordeaux</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Olabarrieta, I" sort="Olabarrieta, I" uniqKey="Olabarrieta I" first="I" last="Olabarrieta">I. Olabarrieta</name></noRegion><name sortKey="Cajaraville, M P" sort="Cajaraville, M P" uniqKey="Cajaraville M" first="M.P" last="Cajaraville">M.P Cajaraville</name><name sortKey="Cajaraville, M P" sort="Cajaraville, M P" uniqKey="Cajaraville M" first="M.P" last="Cajaraville">M.P Cajaraville</name></country><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="L Azou, B" sort="L Azou, B" uniqKey="L Azou B" first="B" last="L'Azou">B. L'Azou</name></region><name sortKey="Cambar, J" sort="Cambar, J" uniqKey="Cambar J" first="J" last="Cambar">J. Cambar</name><name sortKey="Yuric, S" sort="Yuric, S" uniqKey="Yuric S" first="S" last="Yuric">S. Yuric</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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